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PRAGMIN Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87473

Novus Biologicals, part of Bio-Techne
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NBP1-87473
NBP1-87473-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TNGVAISDPSRCPQPAASSASEQRRPRFQAGTWSRQCRIEEEEEVEQELLSHSWGRETKNGPTDHSNSTTWHRLHPTDGSSGQNSKVGTGMSKSASFAFEFPKD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRAGMIN Antibody

Western Blot: PRAGMIN Antibody [NBP1-87473]

Western Blot: PRAGMIN Antibody [NBP1-87473]

Western Blot: PRAGMIN Antibody [NBP1-87473] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: PRAGMIN Antibody [NBP1-87473]

Immunocytochemistry/ Immunofluorescence: PRAGMIN Antibody [NBP1-87473]

Immunocytochemistry/Immunofluorescence: PRAGMIN Antibody [NBP1-87473] - Staining of human cell line U-251 MG shows positivity in cytoplasm, centrosome & nucleus but excluded from the nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PRAGMIN Antibody [NBP1-87473]

Immunohistochemistry-Paraffin: PRAGMIN Antibody [NBP1-87473]

Immunohistochemistry-Paraffin: PRAGMIN Antibody [NBP1-87473] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.

Applications for PRAGMIN Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRAGMIN

Alternate Names

DKFZp761P0423, homolog of rat pragma of Rnd2, PRAGMIN, sugen kinase 223, tyrosine-protein kinase SgK223

Gene Symbol

SGK223

Additional PRAGMIN Products

Product Documents for PRAGMIN Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PRAGMIN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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