PRAGMIN Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87473
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TNGVAISDPSRCPQPAASSASEQRRPRFQAGTWSRQCRIEEEEEVEQELLSHSWGRETKNGPTDHSNSTTWHRLHPTDGSSGQNSKVGTGMSKSASFAFEFPKD
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PRAGMIN Antibody
Western Blot: PRAGMIN Antibody [NBP1-87473]
Western Blot: PRAGMIN Antibody [NBP1-87473] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: PRAGMIN Antibody [NBP1-87473]
Immunocytochemistry/Immunofluorescence: PRAGMIN Antibody [NBP1-87473] - Staining of human cell line U-251 MG shows positivity in cytoplasm, centrosome & nucleus but excluded from the nucleoli. Antibody staining is shown in green.Immunohistochemistry-Paraffin: PRAGMIN Antibody [NBP1-87473]
Immunohistochemistry-Paraffin: PRAGMIN Antibody [NBP1-87473] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.Applications for PRAGMIN Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PRAGMIN
Alternate Names
DKFZp761P0423, homolog of rat pragma of Rnd2, PRAGMIN, sugen kinase 223, tyrosine-protein kinase SgK223
Gene Symbol
SGK223
Additional PRAGMIN Products
Product Documents for PRAGMIN Antibody
Product Specific Notices for PRAGMIN Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...