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PRAK Antibody (2D5)

Novus Biologicals, part of Bio-Techne | Catalog # H00008550-M01

Novus Biologicals, part of Bio-Techne
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H00008550-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2D5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

MAPKAPK5 (AAH47284, 371 a.a. ~ 471 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KDSVYIHDHENGAEDSNVALEKLRDVIAQCILPQAGENEDEKLNEVMQEAWKYNRECKLLRDTLQSFSWNGRGFTDKVDRLKLAEIVKQVIEEQTTSHESQ

Specificity

MAPKAPK5 (2D5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PRAK Antibody (2D5)

Western Blot: PRAK Antibody (2D5) [H00008550-M01]

Western Blot: PRAK Antibody (2D5) [H00008550-M01]

Western Blot: PRAK Antibody (2D5) [H00008550-M01] - Analysis of MAPKAPK5 expression in transfected 293T cell line by MAPKAPK5 monoclonal antibody (M01), clone 2D5.Lane 1: MAPKAPK5 transfected lysate(54 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: PRAK Antibody (2D5) [H00008550-M01] - Detection limit for recombinant GST tagged MAPKAPK5 is approximately 0.1ng/ml as a capture antibody.

Applications for PRAK Antibody (2D5)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein on ELISA. GST alone used as a negative control. It has been used for WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PRAK

The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.

Alternate Names

EC 2.7.11, MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK-5, mitogen-activated protein kinase-activated protein kinase 5, p38-regulated/activated protein kinase, PRAKEC 2.7.11.1

Entrez Gene IDs

8550 (Human)

Gene Symbol

MAPKAPK5

OMIM

606723 (Human)

Additional PRAK Products

Product Documents for PRAK Antibody (2D5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PRAK Antibody (2D5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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