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PRAK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33093

Novus Biologicals, part of Bio-Techne
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NBP1-33093

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (98%), Chicken (97%), Monkey (99%), Mouse (98%), Porcine (98%), Rat (97%), Zebrafish (93%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRAK. The exact sequence is proprietary.

Reactivity Notes

Rhesus Monkey (99%).

Localization

Cytoplasm (Probable), Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PRAK Antibody

Western Blot: PRAK Antibody [NBP1-33093]

Western Blot: PRAK Antibody [NBP1-33093]

Western Blot: PRAK Antibody [NBP1-33093] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE; antibody diluted at 1:1000.

Applications for PRAK Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PRAK

The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.

Alternate Names

EC 2.7.11, MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK-5, mitogen-activated protein kinase-activated protein kinase 5, p38-regulated/activated protein kinase, PRAKEC 2.7.11.1

Gene Symbol

MAPKAPK5

Additional PRAK Products

Product Documents for PRAK Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PRAK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.