PRAK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87231

Novus Biologicals, part of Bio-Techne
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NBP1-87231
NBP1-87231-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PRAK Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PLHSVNNPILRKRKLLGTKPKDSVYIHDHENGAEDSNVALEKLRDVIAQCILPQAGKGENEDEKLNEVMQEAWKYNRECKLLRDTLQSFSWNGRGFTDKVDRLKLAEIVKQVIEEQTTSH

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRAK Antibody

Immunocytochemistry/ Immunofluorescence: PRAK Antibody [NBP1-87231]

Immunocytochemistry/ Immunofluorescence: PRAK Antibody [NBP1-87231]

Immunocytochemistry/Immunofluorescence: PRAK Antibody [NBP1-87231] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: PRAK Antibody [NBP1-87231]

Immunohistochemistry-Paraffin: PRAK Antibody [NBP1-87231]

Immunohistochemistry-Paraffin: PRAK Antibody [NBP1-87231] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: PRAK Antibody [NBP1-87231]

Immunohistochemistry-Paraffin: PRAK Antibody [NBP1-87231]

Immunohistochemistry-Paraffin: PRAK Antibody [NBP1-87231] - Staining of human rectum shows strong nuclear positivity in glandular cells.
View 2 more images

Applications for PRAK Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRAK

PRAK is encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.

Alternate Names

EC 2.7.11, MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK-5, mitogen-activated protein kinase-activated protein kinase 5, p38-regulated/activated protein kinase, PRAKEC 2.7.11.1

Gene Symbol

MAPKAPK5

Additional PRAK Products

Product Documents for PRAK Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PRAK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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