PRC1 Antibody (16F2)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-74508
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 16F2
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant PRC1 protein.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
Nuclear, depending on the phase of the cell cycle.
Specificity
PRC1 (16F2)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for PRC1 Antibody (16F2)
Immunocytochemistry/ Immunofluorescence: PRC1 Antibody (16F2) [NB100-74508]
Immunocytochemistry/Immunofluorescence: PRC1 Antibody (16F2) [NB100-74508] - Analysis of PRC1 using PRC1 Monoclonal Antibody (16F2) shows staining in U251 Cells. PRC1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing PRC1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.Immunocytochemistry/ Immunofluorescence: PRC1 Antibody (16F2) [NB100-74508]
Immunocytochemistry/Immunofluorescence: PRC1 Antibody (16F2) [NB100-74508] - Analysis of PRC1 using PRC1 Monoclonal Antibody (16F2) shows staining in A2058 Cells. PRC1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing PRC1 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.Immunocytochemistry/ Immunofluorescence: PRC1 Antibody (16F2) [NB100-74508]
Immunocytochemistry/Immunofluorescence: PRC1 Antibody (16F2) [NB100-74508] - Analysis of PRC1 using PRC1 Monoclonal Antibody (16F2) shows staining in Hela Cells. PRC1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing PRC1 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.Applications for PRC1 Antibody (16F2)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1 - 2 ug/ml
Immunoprecipitation
1:10 - 1:500
Western Blot
1 - 3 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PRC1
Alternate Names
anaphase spindle elongation 1 homolog, ASE1, MGC1671, MGC3669, protein regulating cytokinesis 1, protein regulator of cytokinesis 1
Gene Symbol
PRC1
UniProt
Additional PRC1 Products
Product Documents for PRC1 Antibody (16F2)
Product Specific Notices for PRC1 Antibody (16F2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...