PRC1 Antibody (SR2177)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21612

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21612-100ul
NBP3-21612-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2177

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PRC1 Antibodies »

Product Summary for PRC1 Antibody (SR2177)

Immunogen

A synthesized peptide derived from human PRC1 (Uniprot #: O43663)

Specificity

Detects endogenous levels of total PRC1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PRC1 Antibody (SR2177)

Western Blot: PRC1 Antibody (SR2177) [NBP3-21612] -

Western Blot: PRC1 Antibody (SR2177) [NBP3-21612] - Western blot analysis using NBP3-21612 in HeLa cell lysate.

Applications for PRC1 Antibody (SR2177)

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRC1

Novel human protein Protein regulator of cytokinesis 1 (PRC1) is involved in cytokinesis. PRC1 is a good substrate for several CDKs in vitro and is phosphorylated in vivo at sites that are phosphorylated by CDK in vitro, strongly suggesting that PRC1 is an in vivo CDK substrate. PRC1 protein levels are high during S and G2/M and drop dramatically after cells exit mitosis and enter G1. PRC1 is a nuclear protein in interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis (1). PRC1, a mitotic spindle-associated Cdk substrate that is essential to cell cleavage, is a microtubule binding and bundling protein both in vivo and in vitro. Over expression of PRC1 extensively bundles interphase microtubules, but does not affect early mitotic spindle organization (2). PRC1 has been shown to down-regulate the GAP activity of MgcRac-GAP during the metaphase and thereby contributes to the correct formation of the spindle (3).

Alternate Names

anaphase spindle elongation 1 homolog, ASE1, MGC1671, MGC3669, protein regulating cytokinesis 1, protein regulator of cytokinesis 1

Gene Symbol

PRC1

Additional PRC1 Products

Product Documents for PRC1 Antibody (SR2177)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PRC1 Antibody (SR2177)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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