PRC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19925

Novus Biologicals, part of Bio-Techne
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NBP2-19925
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PRC1 Antibodies »

Product Summary for PRC1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PRC1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (81%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PRC1 Antibody

Western Blot: PRC1 Antibody [NBP2-19925]

Western Blot: PRC1 Antibody [NBP2-19925]

Western Blot: PRC1 Antibody [NBP2-19925] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PRC1 antibody [N1N2], N-term diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PRC1 Antibody [NBP2-19925]

Immunocytochemistry/ Immunofluorescence: PRC1 Antibody [NBP2-19925]

Immunocytochemistry/Immunofluorescence: PRC1 Antibody [NBP2-19925] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: PRC1 Antibody [NBP2-19925]

Immunohistochemistry-Paraffin: PRC1 Antibody [NBP2-19925]

Immunohistochemistry-Paraffin: PRC1 Antibody [NBP2-19925] - SCM1 xenograft, using PRC1antibody at 1:100 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min
View 1 more images

Applications for PRC1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PRC1

This gene encodes a protein that is involved in cytokinesis. The encoded protein is at high level during S and G2/M and drop dramatically after cell exit mitosis and enter G1. It is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Alternate Names

anaphase spindle elongation 1 homolog, ASE1, MGC1671, MGC3669, protein regulating cytokinesis 1, protein regulator of cytokinesis 1

Gene Symbol

PRC1

UniProt

O43663 (Human)

Additional PRC1 Products

Product Documents for PRC1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PRC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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