PRC1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81882

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all PRC1 Antibodies »

Product Summary for PRC1 Antibody - BSA Free

Immunogen

PRC1 antibody was raised against a 19 amino acid synthetic peptide near the center of human PRC1. The immunogen is located within amino acids 350 - 400 of PRC1. Amino Acid Squence: LEFERKASDPNRFTNRGGN

Specificity

At least three alternatively spliced transcript variants encoding distinct isoforms have been observed.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRC1 Antibody - BSA Free

Western Blot: PRC1 AntibodyBSA Free [NBP2-81882]

Western Blot: PRC1 AntibodyBSA Free [NBP2-81882]

Western Blot: PRC1 Antibody [NBP2-81882] - Analysis of PRC1 in human skeletal muscle tissue lysate with PRC1 antibody at (A) 0.5 ug/ml and (B) 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: PRC1 Antibody - BSA Free [NBP2-81882]

Immunocytochemistry/ Immunofluorescence: PRC1 Antibody - BSA Free [NBP2-81882]

Immunocytochemistry/Immunofluorescence: PRC1 Antibody [NBP2-81882] - Analysis of PRC1 in mouse skeletal muscle tissue with PRC1 antibody at 20 ug/ml.
Immunohistochemistry: PRC1 Antibody - BSA Free [NBP2-81882]

Immunohistochemistry: PRC1 Antibody - BSA Free [NBP2-81882]

Immunohistochemistry: PRC1 Antibody [NBP2-81882] - Analysis of PRC1 in mouse skeletal muscle tissue with PRC1 antibody at 2.5 ug/ml.

Applications for PRC1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2.5 ug/ml

Immunohistochemistry

2.5 ug/ml

Western Blot

0.5-1 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRC1

Novel human protein Protein regulator of cytokinesis 1 (PRC1) is involved in cytokinesis. PRC1 is a good substrate for several CDKs in vitro and is phosphorylated in vivo at sites that are phosphorylated by CDK in vitro, strongly suggesting that PRC1 is an in vivo CDK substrate. PRC1 protein levels are high during S and G2/M and drop dramatically after cells exit mitosis and enter G1. PRC1 is a nuclear protein in interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis (1). PRC1, a mitotic spindle-associated Cdk substrate that is essential to cell cleavage, is a microtubule binding and bundling protein both in vivo and in vitro. Over expression of PRC1 extensively bundles interphase microtubules, but does not affect early mitotic spindle organization (2). PRC1 has been shown to down-regulate the GAP activity of MgcRac-GAP during the metaphase and thereby contributes to the correct formation of the spindle (3).

Alternate Names

anaphase spindle elongation 1 homolog, ASE1, MGC1671, MGC3669, protein regulating cytokinesis 1, protein regulator of cytokinesis 1

Gene Symbol

PRC1

Additional PRC1 Products

Product Documents for PRC1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PRC1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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