PRDM5 Antibody (7D4C12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52540

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7D4C12

Format

BSA Free

Concentration

1 mg/ml

View all PRDM5 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human PRDM5 (AA: 1-100) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PRDM5 Antibody (7D4C12) - BSA Free

Western Blot: PRDM5 Antibody (7D4C12)BSA Free [NBP2-52540]

Western Blot: PRDM5 Antibody (7D4C12)BSA Free [NBP2-52540]

Western Blot: PRDM5 Antibody (7D4C12) [NBP2-52540] - Western blot analysis using PRDM5 mouse mAb against HL-60 (1), NIH/3T3 (2), SW480 (3), and HEK293 (4) cell lysate.
Immunocytochemistry/ Immunofluorescence: PRDM5 Antibody (7D4C12) - BSA Free [NBP2-52540]

Immunocytochemistry/ Immunofluorescence: PRDM5 Antibody (7D4C12) - BSA Free [NBP2-52540]

Immunocytochemistry/Immunofluorescence: PRDM5 Antibody (7D4C12) [NBP2-52540] - Analysis of MCF-7 cells using PRDM5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow Cytometry: PRDM5 Antibody (7D4C12) - BSA Free [NBP2-52540]

Flow Cytometry: PRDM5 Antibody (7D4C12) - BSA Free [NBP2-52540]

Flow Cytometry: PRDM5 Antibody (7D4C12) [NBP2-52540] - Analysis of HeLa cells using PRDM5 mouse mAb (green) and negative control (red).
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Applications for PRDM5 Antibody (7D4C12) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 31119897).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRDM5

Official Gene Symbol PRDM5 Gene ID 11107(Human) Gene Map Locus 4q25-q26(Human) PRDM5, a novel member of PR domain protein family, contains a PR-domain at the NH2 terminus followed by 16 zinc finger motifs. It is found to have a role in cell differentiation and tumorigenesis. Reports suggest PRDM5 causes G2/M arrest and apoptosis upon infection of tumor cells and has been found to be silenced in breast, ovarian and lung cancers, suggesting a potential clinical significance.

Alternate Names

PFM2PR domain-containing protein 5, PR domain containing 5, PR domain zinc finger protein 5

Gene Symbol

PRDM5

Additional PRDM5 Products

Product Documents for PRDM5 Antibody (7D4C12) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PRDM5 Antibody (7D4C12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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