PRDM9 Antibody (1H9Z1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35059
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Serum, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 1H9Z1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 87-370 of mouse PRDM9 (NP_659058.3).
Sequence:
DSEDSDEEWTPKQQVSPPWVPFRVKHSKQQKESSRMPFSGESNVKEGSGIENLLNTSGSEHVQKPVSSLEEGNTSGQHSGKKLKLRKKNVEVKMYRLRERKGLAYEEVSEPQDDDYLYCEKCQNFFIDSCPNHGPPLFVKDSMVDRGHPNHSVLSLPPGLRISPSGIPEAGLGVWNEASDLPVGLHFGPYEGQITEDEEAANSGYSWLITKGRNCYEYVDGQDESQANWMRYVNCARDDEEQNLVAFQYHRKIFYRTCRVIRPGCELLVWYGDEYGQELGIKWG
Sequence:
DSEDSDEEWTPKQQVSPPWVPFRVKHSKQQKESSRMPFSGESNVKEGSGIENLLNTSGSEHVQKPVSSLEEGNTSGQHSGKKLKLRKKNVEVKMYRLRERKGLAYEEVSEPQDDDYLYCEKCQNFFIDSCPNHGPPLFVKDSMVDRGHPNHSVLSLPPGLRISPSGIPEAGLGVWNEASDLPVGLHFGPYEGQITEDEEAANSGYSWLITKGRNCYEYVDGQDESQANWMRYVNCARDDEEQNLVAFQYHRKIFYRTCRVIRPGCELLVWYGDEYGQELGIKWG
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PRDM9 Antibody (1H9Z1)
Immunohistochemistry: PRDM9 Antibody (1H9Z1) [NBP3-35059] -
Immunohistochemistry: PRDM9 Antibody (1H9Z1) [NBP3-35059] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using PRDM9 Rabbit mAb at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry: PRDM9 Antibody (1H9Z1) [NBP3-35059] -
Immunohistochemistry: PRDM9 Antibody (1H9Z1) [NBP3-35059] - Immunohistochemistry analysis of paraffin-embedded Human colon using PRDM9 Rabbit mAb at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Western Blot: PRDM9 Antibody (1H9Z1) [NBP3-35059] -
Western Blot: PRDM9 Antibody (1H9Z1) [NBP3-35059] - Western blot analysis of lysates from Rat kidney, using PRDM9 Rabbit mAb at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Applications for PRDM9 Antibody (1H9Z1)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:500 - 1:1000
Serum
CUT&Tag 105 cells /1 ug
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PRDM9
Long Name
Histone-lysine N-methyltransferase PRDM9
Alternate Names
EC 2.1.1.-, EC 2.1.1.354, EC 2.1.1.359, EC 2.1.1.361, EC 2.1.1.362, PFM6
Additional PRDM9 Products
Product Specific Notices for PRDM9 Antibody (1H9Z1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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