PRDM9 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16014
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Chromatin Immunoprecipitation (ChIP), Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 87-370 of mouse Prdm9 (NP_659058.3). DSEDSDEEWTPKQQVSPPWVPFRVKHSKQQKESSRMPFSGESNVKEGSGIENLLNTSGSEHVQKPVSSLEEGNTSGQHSGKKLKLRKKNVEVKMYRLRERKGLAYEEVSEPQDDDYLYCEKCQNFFIDSCPNHGPPLFVKDSMVDRGHPNHSVLSLPPGLRISPSGIPEAGLGVWNEASDLPVGLHFGPYEGQITEDEEAANSGYSWLITKGRNCYEYVDGQDESQANWMRYVNCARDDEEQNLVAFQYHRKIFYRTCRVIRPGCELLVWYGDEYGQELGIKWG
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PRDM9 Antibody - Azide and BSA Free
![Western Blot: PRDM9 AntibodyAzide and BSA Free [NBP3-16014]](https://resources.bio-techne.com/images/products/PRDM9-Antibody-Western-Blot-NBP3-16014-img0002.jpg "Western Blot: PRDM9 AntibodyAzide and BSA Free [NBP3-16014]")
Western Blot: PRDM9 AntibodyAzide and BSA Free [NBP3-16014]
Western Blot: PRDM9 Antibody [NBP3-16014] - Analysis of extracts of various cell lines, using PRDM9 antibody (NBP3-16014) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Chromatin Immunoprecipitation: PRDM9 Antibody [NBP3-16014] - Chromatin immunoprecipitation analysis of extracts of Mouse testis, using PRDM9 antibody (NBP3-16014) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
![Western Blot: PRDM9 AntibodyAzide and BSA Free [NBP3-16014]](https://resources.bio-techne.com/images/products/PRDM9-Antibody-Western-Blot-NBP3-16014-img0001.jpg "Western Blot: PRDM9 AntibodyAzide and BSA Free [NBP3-16014]")
Western Blot: PRDM9 AntibodyAzide and BSA Free [NBP3-16014]
Western Blot: PRDM9 Antibody [NBP3-16014] - Analysis of extracts of various cell lines, using PRDM9 antibody (NBP3-16014) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Applications for PRDM9 Antibody - Azide and BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 50% glycerol, pH7.3
Format
Azide and BSA Free
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PRDM9
Long Name
Histone-lysine N-methyltransferase PRDM9
Alternate Names
EC 2.1.1.-, EC 2.1.1.354, EC 2.1.1.359, EC 2.1.1.361, EC 2.1.1.362, PFM6
Gene Symbol
PRDM9
Additional PRDM9 Products
Product Documents for PRDM9 Antibody - Azide and BSA Free
Product Specific Notices for PRDM9 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...