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pre-mRNA cleavage factor I (59 kDa subunit) Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89868

Novus Biologicals, part of Bio-Techne
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NBP1-89868

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SDDRSSSTEPPPPVRQEPSPKPNNKTPAILYTYSGLRNRRAAVYVGSFSWWTTDQQLIQVIRSIGVYDVVELKFAENRA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for pre-mRNA cleavage factor I (59 kDa subunit) Antibody

Western Blot: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NBP1-89868]

Western Blot: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NBP1-89868]

Western Blot: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NBP1-89868] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NBP1-89868]

Immunocytochemistry/ Immunofluorescence: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NBP1-89868]

Immunocytochemistry/Immunofluorescence: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NBP1-89868] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NBP1-89868]

Immunohistochemistry-Paraffin: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NBP1-89868]

Immunohistochemistry-Paraffin: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NBP1-89868] - Staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.

Applications for pre-mRNA cleavage factor I (59 kDa subunit) Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: pre-mRNA cleavage factor I (59 kDa subunit)

Alternate Names

CFIm59, cleavage and polyadenylation specific factor 7, 59kDa, CPSF 59 kDa subunit, FLJ12529,59 kDa subunit, FLJ39024, MGC9315, pre-mRNA cleavage factor I, 59 kDa subunit

Gene Symbol

CPSF7

Additional pre-mRNA cleavage factor I (59 kDa subunit) Products

Product Documents for pre-mRNA cleavage factor I (59 kDa subunit) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for pre-mRNA cleavage factor I (59 kDa subunit) Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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