Pref-1/DLK1/FA1 Antibody (PSH04-76)

Western Blot: Pref-1/DLK1/FA1 Antibody (PSH04-76) [NBP3-32270]

Western Blot: Pref-1/DLK1/FA1 Antibody (PSH04-76) [NBP3-32270] - Western blot analysis of Pref-1/DLK1/FA1 on different lysates with Rabbit anti-Pref-1/DLK1/FA1 antibody (NBP3-32270) at 1/1,000 dilution.

Lane 1: MCF7 cell lysate (20 ug/Lane)
Lane 2: HepG2 cell lysate (20 ug/Lane)
Lane 3: SH-SY5Y cell lysate (20 ug/Lane)
Lane 4: Mouse placenta tissue lysate (70 heat) (40 ug/Lane)
Lane 5: Rat embryo tissue lysate (40 ug/Lane)

Predicted band size: 41 kDa
Observed band size: 40-50kDa

Exposure time: Lane 1-4: 2 minutes; Lane 5: 27 seconds; ECL;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32270) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Pref-1/DLK1/FA1 Antibody (PSH04-76) [NBP3-32270]

Immunohistochemistry: Pref-1/DLK1/FA1 Antibody (PSH04-76) [NBP3-32270] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Pref-1/DLK1/FA1 antibody (NBP3-32270) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32270) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.