preproANP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14873

Novus Biologicals, part of Bio-Techne
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NBP2-14873
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all preproANP Antibodies »

Product Specifications

Immunogen

Full length human preproANP Recombinant protein.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (83%), Porcine (87%), Rabbit (81%), Bovine (83%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for preproANP Antibody

Western Blot: preproANP Antibody [NBP2-14873]

Western Blot: preproANP Antibody [NBP2-14873]

Western Blot: preproANP Antibody [NBP2-14873] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody [N1C3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14873]

Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14873]

Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14873] - Mouse muscle. ANP stained by ANP antibody [N1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: preproANP Antibody [NBP2-14873]

Western Blot: preproANP Antibody [NBP2-14873]

Western Blot: preproANP Antibody [NBP2-14873] - Knockdown of ATE1 attenuates cardiac hypertrophy. Western blot analysis of preproANP protein levels in heart samples from sham and renal ligated rats (B2) bar graph was generated by quantifying the blots and normalizing the intensities of bands to the untreated lane (C) BNP and (D) beta-MHC in Ligated ATE1 siRNA when compared with Ligated NS siRNA samples. Statistical analysis was carried out by one-way ANOVA. Data are represented as mean +/- SE. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-019-57379-7) licensed under a CC-BY license.
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Applications for preproANP Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: preproANP

The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. This gene is located adjacent to another member of the natriuretic family of peptides on chromosome 1.

Alternate Names

ANF, ANP, ATFB6, atrial natriuretic factor prohormone, atrial natriuretic peptide prohormone, ATRST2, CDD, CDD-ANF, CDP, natriuretic peptides A, PND

Gene Symbol

NPPA

UniProt

P01160

Additional preproANP Products

Product Documents for preproANP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for preproANP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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