Presenilin-1 Antibody (3W4V8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15665

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15665-100ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3W4V8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Presenilin-1 Antibodies »

Product Summary for Presenilin-1 Antibody (3W4V8)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Presenilin-1 (P49768). MTELPAPLSYFQNAQMSEDNHLSNTVRSQNDNRERQEHNDRRSLGHPEPLSNGRPQGNSRQVVEQDEEEDEELTLKYGAKHVIMLFVPVTLCMVVVVATI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Presenilin-1 Antibody (3W4V8)

Western Blot: Presenilin-1 Antibody (3W4V8) [NBP3-15665]

Western Blot: Presenilin-1 Antibody (3W4V8) [NBP3-15665]

Western Blot: Presenilin-1 Antibody (3W4V8) [NBP3-15665] - Western blot analysis of extracts of various cell lines, using Presenilin-1 antibody (NBP3-15665) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Applications for Presenilin-1 Antibody (3W4V8)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Presenilin-1

Presenilin (PS) facilitates gamma-secretase cleavage of the beta-amyloid precursor protein and the intramembraneous cleavage of Notch1 (1). Presenilin (PS) is essential for the gamma-cleavage required for the generation of the C terminus of amyloid beta-protein (Abeta). The discovery that a deficiency of presenilin 1 (PS1) decreases the production of amyloid beta-protein (Abeta) identified the presenilins as important mediators of the gamma-secretase cleavage of beta-amyloid precursor protein (APP). It has been shown that two conserved transmembrane (TM) aspartates in PS1 are critical for Abeta production, providing evidence that PS either functions as a required diaspartyl cofactor for gamma-secretase or is itself gamma-secretase. Presenilin 2 (PS2) shares substantial sequence and possibly functional homology with PS1. It has been shown that the two TM aspartates in PS2 are also critical for gamma-secretase activity, providing further evidence that PS2 is functionally homologous to PS1 (2). PS are found primarily within intracellular membranes, including the endoplasmic reticulum (ER) and the trans-Golgi network as well as the plasma membrane. PS are also expressed in most cell types throughout development (3).

Alternate Names

Presenilin1, PSEN1, S182

Gene Symbol

PSEN1

Additional Presenilin-1 Products

Product Documents for Presenilin-1 Antibody (3W4V8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Presenilin-1 Antibody (3W4V8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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