PRKAG2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32697

Novus Biologicals, part of Bio-Techne
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NBP1-32697
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Chicken (91%), Mouse (98%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PRKAG2 Antibodies »

Product Summary for PRKAG2 Antibody

Immunogen

Full length human PRKAG2 Recombinant protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PRKAG2 Antibody

Western Blot: PRKAG2 Antibody [NBP1-32697]

Western Blot: PRKAG2 Antibody [NBP1-32697]

Western Blot: PRKAG2 Antibody [NBP1-32697] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with AMPK gamma 2 antibody at a dilution of 1:1000.
Immunocytochemistry/ Immunofluorescence: PRKAG2 Antibody [NBP1-32697]

Immunocytochemistry/ Immunofluorescence: PRKAG2 Antibody [NBP1-32697]

Immunocytochemistry/Immunofluorescence: PRKAG2 Antibody [NBP1-32697] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-32697]

Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-32697]

Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-32697] - Gastric cancer tissue. AMPK gamma 2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for PRKAG2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PRKAG2

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family and encodes a protein with four cystathionine beta-synthase domains. Mutations in this gene have been associated with ventricular pre-excitation (Wolff-Parkinson-White syndrome), progressive conduction system disease and cardiac hypertrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Long Name

5'-AMP-activated protein kinase subunit gamma-2

Alternate Names

AAKG, AAKG2, AMPK gamma2, CMH6, H91620p, WPWS

Gene Symbol

PRKAG2

Additional PRKAG2 Products

Product Documents for PRKAG2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PRKAG2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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