PRKAG3 Antibody (2C7A7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61796

Novus Biologicals, part of Bio-Techne
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NBP2-61796
NBP2-61796-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2C7A7

Format

BSA Free

Concentration

1 mg/ml

View all AMPK gamma 3 Antibodies »

Product Summary for PRKAG3 Antibody (2C7A7) - BSA Free

Immunogen

Purified recombinant fragment of human PRKAG3 (AA: 9-151) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

54.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PRKAG3 Antibody (2C7A7) - BSA Free

Western Blot: PRKAG3 Antibody (2C7A7)BSA Free [NBP2-61796]

Western Blot: PRKAG3 Antibody (2C7A7)BSA Free [NBP2-61796]

Western Blot: PRKAG3 Antibody (2C7A7) [NBP2-61796] - Analysis using PRKAG3 mAb against human PRKAG3 (AA: 9-151) recombinant protein. (Expected MW is 41.1 kDa)
Immunocytochemistry/ Immunofluorescence: PRKAG3 Antibody (2C7A7) - BSA Free [NBP2-61796]

Immunocytochemistry/ Immunofluorescence: PRKAG3 Antibody (2C7A7) - BSA Free [NBP2-61796]

Immunocytochemistry/Immunofluorescence: PRKAG3 Antibody (2C7A7) [NBP2-61796] - Analysis of Hela cells using PRKAG3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry: PRKAG3 Antibody (2C7A7) - BSA Free [NBP2-61796]

Immunohistochemistry: PRKAG3 Antibody (2C7A7) - BSA Free [NBP2-61796]

Immunohistochemistry: PRKAG3 Antibody (2C7A7) [NBP2-61796] - Analysis of paraffin-embedded rectum tissues using PRKAG3 mouse mAb with DAB staining.
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Applications for PRKAG3 Antibody (2C7A7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AMPK gamma 3

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle.

Long Name

AMP-activated Protein Kinase gamma 3

Alternate Names

AMPKG3, PRKAG3

Gene Symbol

PRKAG3

Additional AMPK gamma 3 Products

Product Documents for PRKAG3 Antibody (2C7A7) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PRKAG3 Antibody (2C7A7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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