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PRKCBP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18649

Novus Biologicals, part of Bio-Techne
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NBP3-18649-100ul
NBP3-18649-25ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Polyclonal antibody raised in rabbit against human PRKCBP1 (zinc finger, MYND-type containing 8), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for PRKCBP1 Antibody

Western Blot: PRKCBP1 Antibody [NBP3-18649]

Western Blot: PRKCBP1 Antibody [NBP3-18649]

Western Blot: PRKCBP1 Antibody [NBP3-18649] - Figure 2. Western blot analysis using the antibody directed against PRKCBP1 Nuclear extracts from HeLa cells (40 ug) were analysed by Western blot using the antibody against PRKCBP1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 132 kDa); the marker (in kDa) is shown on the left.
ELISA: PRKCBP1 Antibody [NBP3-18649]

ELISA: PRKCBP1 Antibody [NBP3-18649]

ELISA: PRKCBP1 Antibody [NBP3-18649] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human PRKCBP1 . The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:36,000.

Applications for PRKCBP1 Antibody

Application
Recommended Usage

ELISA

1:100-1:500

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Whole Antiserum

Formulation

Whole antiserum

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: PRKCBP1

PRKCBP1 is encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

ESP-2, HED-2, zyxin, zyxin-2

Gene Symbol

ZMYND8

UniProt

Additional PRKCBP1 Products

Product Documents for PRKCBP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PRKCBP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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