PRKD3/nPKC nu Antibody (4G7)

Novus Biologicals, part of Bio-Techne | Catalog # H00023683-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4G7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PRKD3/nPKC nu Antibodies »

Product Specifications

Immunogen

PRKD3 (AAH30706, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSANNSPPSAQKSVLPTAIPAVLPAASPCSSPKTGLSARLSNGSFSAPSLTNSRGSVHTVSFLLQIGLTRESVTIEAQELSLSAVKDLVCSIVYQKFPEC

Localization

Cytoplasm, nucleus.

Specificity

PRKD3 (4G7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PRKD3/nPKC nu Antibody (4G7)

ELISA: PRKD3/nPKC nu Antibody (4G7) [H00023683-M01]

ELISA: PRKD3/nPKC nu Antibody (4G7) [H00023683-M01]

ELISA: PRKD3/nPKC nu Antibody (4G7) [H00023683-M01] - Detection limit for recombinant GST tagged PRKD3 is approximately 1ng/ml as a capture antibody.

Applications for PRKD3/nPKC nu Antibody (4G7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
This antibody is reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PRKD3/nPKC nu

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members.

Long Name

Protein Kinase D3

Alternate Names

EPK2, nPKC nu, PKC nu, PRKCN

Entrez Gene IDs

23683 (Human)

Gene Symbol

PRKD3

OMIM

607077 (Human)

UniProt

AAH30706 (Human)

Additional PRKD3/nPKC nu Products

Product Documents for PRKD3/nPKC nu Antibody (4G7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PRKD3/nPKC nu Antibody (4G7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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