PRMT1 Antibody (PRMT1-171) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB120-12189
Key Product Details
Species Reactivity
Human, Mouse, Rat, Bovine, Canine, Chicken, Hamster, Primate
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # PRMT1-171
Format
BSA Free
Concentration
2.0 mg/ml
Product Specifications
Immunogen
Monoclonal Anti-PRMT1 is derived from the PRMT1-171 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with full length PRMT1.
Reactivity Notes
Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
Nuclear
Specificity
This reacts with PRMT1 and does not cross-react with PRMT2, 3, 4, and 5.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
~42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PRMT1 Antibody (PRMT1-171) - BSA Free
![Western Blot: PRMT1 Antibody (PRMT1-171)BSA Free [NB120-12189]](https://resources.bio-techne.com/images/products/PRMT1-Antibody-PRMT1-171-BSA-Free-Western-Blot-NB120-12189-img0003.jpg "Western Blot: PRMT1 Antibody (PRMT1-171)BSA Free [NB120-12189]")
Western Blot: PRMT1 Antibody (PRMT1-171)BSA Free [NB120-12189]
Western Blot: PRMT1 Antibody (PRMT1-171) - BSA Free [NB120-12189] - Cell line lysates were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-PRMT1 Clone: PRMT1-171. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. G361 4. NRK 5. MDBK 6. MDCK 7. CHO 8. 3T3Applications for PRMT1 Antibody (PRMT1-171) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 2 ug/ml (determined by using a whole cell extract of the human kidney cell line 293T). Predicted molecular weight: 42 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
2.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PRMT1
Long Name
Protein Arginine Methyltransferase 1
Alternate Names
ANM1, HCP1, HMT1, HMT2, HRMT1L2, IR1B4
Gene Symbol
PRMT1
Additional PRMT1 Products
Product Documents for PRMT1 Antibody (PRMT1-171) - BSA Free
Product Specific Notices for PRMT1 Antibody (PRMT1-171) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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