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PRMT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2251

Novus Biologicals, part of Bio-Techne
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NB100-2251

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 300 and the C-terminus (residue 361) of human Protein Arginine N-Methyltransferase 1 using the numbering given in entry NP_001527.2 (GeneID 3276).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRMT1 Antibody

Western Blot: PRMT1 Antibody [NB100-2251]

Western Blot: PRMT1 Antibody [NB100-2251]

Western Blot: PRMT1 Antibody [NB100-2251] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-PRMT1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: PRMT1 Antibody [NB100-2251]

Immunohistochemistry-Paraffin: PRMT1 Antibody [NB100-2251]

Immunohistochemistry-Paraffin: PRMT1 Antibody [NB100-2251] - Section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- PRMT1 used at a dilution of 1:200 (1 ug/ml). Detection: DAB
Western Blot: PRMT1 Antibody [NB100-2251]

Western Blot: PRMT1 Antibody [NB100-2251]

Western Blot: PRMT1 Antibody [NB100-2251] - Detection of Human and Mouse PRMT1 on HeLa whole cell lysate using NB100-2251. PRMT1 was also immunoprecipitated using rabbit anti-PRMT1 antibody NB100-2250.

Applications for PRMT1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PRMT1

The HRMT1L2 gene encodes a protein arginine methyltransferase that functions as a histone methyltransferase specific for H4 (see MIM 602822).[supplied by OMIM]

Long Name

Protein Arginine Methyltransferase 1

Alternate Names

ANM1, HCP1, HMT1, HMT2, HRMT1L2, IR1B4

Entrez Gene IDs

3276 (Human); 15469 (Mouse)

Gene Symbol

PRMT1

Additional PRMT1 Products

Product Documents for PRMT1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PRMT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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