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PRMT3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19933

Novus Biologicals, part of Bio-Techne
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NBP2-19933

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (91%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for PRMT3 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRMT3. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PRMT3 Antibody

Western Blot: PRMT3 Antibody [NBP2-19933]

Western Blot: PRMT3 Antibody [NBP2-19933]

Western Blot: PRMT3 Antibody [NBP2-19933] - PRMT3 antibody immunoprecipitates PRMT3 protein in IP experiments. IP samples: HeLa whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of PRMT3 protein by 4 µg PRMT3 antibody. 7.5 % SDS-PAGE. The immunoprecipitated PRMT3 protein was detected by PRMT3 antibody diluted at 1:500.
Western Blot: PRMT3 Antibody [NBP2-19933]

Western Blot: PRMT3 Antibody [NBP2-19933]

Western Blot: PRMT3 Antibody [NBP2-19933] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: PRMT3 Antibody [NBP2-19933]

Western Blot: PRMT3 Antibody [NBP2-19933]

Western Blot: PRMT3 Antibody [NBP2-19933] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:5000.

Applications for PRMT3 Antibody

Application
Recommended Usage

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PRMT3

Type I protein arginine N-methyltransferases (PRMTs), such as PRMT3, catalyze the formation of asymmetric N(G),N(G)-dimethylarginine (ADMA) residues in proteins (Tang et al., 1998 [PubMed 9642256]).[supplied by OMIM]

Long Name

Protein arginine N-methyltransferase 3

Alternate Names

EC 2.1.1.319, HRMT1L3

Gene Symbol

PRMT3

Additional PRMT3 Products

Product Documents for PRMT3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PRMT3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.