PRMT5 Antibody (2H4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26367
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2H4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for PRMT5 Antibody (2H4)
Immunogen
A synthesized peptide derived from Human PRMT5 [UniProt O14744]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PRMT5 Antibody (2H4)
Immunohistochemistry: PRMT5 Antibody (2H4) [NBP3-26367] -
Immunohistochemistry: PRMT5 Antibody (2H4) [NBP3-26367] - Image of PRMT5 Antibody (2H4) diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: PRMT5 Antibody (2H4) [NBP3-26367] -
Western Blot: PRMT5 Antibody (2H4) [NBP3-26367] - Positive Western Blot detected in: Raji whole cell lysate, Hela whole cell lysate, HL60 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate.All lanes: PRMT5 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 73, 72, 54, 68, 67 kDa
Observed band size: 73 kDa
Applications for PRMT5 Antibody (2H4)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: PRMT5
Long Name
Protein arginine N-methyltransferase 5
Alternate Names
EC 2.1.1.320, HRMT1L5, IBP72, JBP1, SKB1, SKB1 homolog
Gene Symbol
PRMT5
Additional PRMT5 Products
Product Documents for PRMT5 Antibody (2H4)
Product Specific Notices for PRMT5 Antibody (2H4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...