PRMT6 Antibody (PCRP-PRMT6-2C9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07656

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-PRMT6-2C9

Concentration

0.2 mg/ml

View all PRMT6 Antibodies »

Product Summary for PRMT6 Antibody (PCRP-PRMT6-2C9)

Immunogen

Recombinant full-length human PRMT6 protein (Uniprot: Q96LA8 )

Reactivity Notes

Predicted to react with Rat.

Localization

Nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08393)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for PRMT6 Antibody (PCRP-PRMT6-2C9)

Immunocytochemistry/ Immunofluorescence: PRMT6 Antibody (PCRP-PRMT6-2C9) [NBP3-07656]

Immunocytochemistry/ Immunofluorescence: PRMT6 Antibody (PCRP-PRMT6-2C9) [NBP3-07656]

Immunocytochemistry/Immunofluorescence: PRMT6 Antibody (PCRP-PRMT6-2C9) [NBP3-07656] - Immunofluorescence Analysis of PFA-fixed MCF7 cells stained using PRMT6 antibody (PCRP-PRMT6-2C9) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).
Flow Cytometry: PRMT6 Antibody (PCRP-PRMT6-2C9) [NBP3-07656]

Flow Cytometry: PRMT6 Antibody (PCRP-PRMT6-2C9) [NBP3-07656]

Flow Cytometry: PRMT6 Antibody (PCRP-PRMT6-2C9) [NBP3-07656] - Flow Cytometric Analysis of PFA-fixed HeLa cells. PRMT6 Mouse Monoclonal Antibody (PCRP-PRMT6-2C9) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).
Protein Array: PRMT6 Antibody (PCRP-PRMT6-2C9) [NBP3-07656]

Protein Array: PRMT6 Antibody (PCRP-PRMT6-2C9) [NBP3-07656]

Protein Array: PRMT6 Antibody (PCRP-PRMT6-2C9) [NBP3-07656] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PRMT6 Mouse Monoclonal Antibody (PCRP-PRMT6-2C9).

Applications for PRMT6 Antibody (PCRP-PRMT6-2C9)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein
Application Notes
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PRMT6

PRMT6 severely impairs the function of HIV-1 Rev. PRMT6 is a protein arginine N-methyltransferase, and catalyzes the sequential transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation is a prevalent post-translational modification in eukaryotic cells that has been implicated in signal transduction, the metabolism of nascent pre-RNA, and the transcriptional activation processes. In addition, PRMT6 displays automethylation activity; it is the first PRMT to do so. PRMT6 has been shown to act as a restriction factor for HIV replication.

Long Name

Protein arginine N-methyltransferase 6

Alternate Names

HRMT1L6

Gene Symbol

PRMT6

UniProt

Q96LA8 (Human)

Additional PRMT6 Products

Product Documents for PRMT6 Antibody (PCRP-PRMT6-2C9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PRMT6 Antibody (PCRP-PRMT6-2C9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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