Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation (Negative), Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PRMT6 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 325 and the C-terminus (residue 375) of human Protein Arginine Methyltransferase 6 using the numbering given in entry AAK85733.1 (GeneID 55170).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRMT6 Antibody

Western Blot: PRMT6 Antibody [NB110-40713]

Western Blot: PRMT6 Antibody [NB110-40713]

Western Blot: PRMT6 Antibody [NB110-40713] - Detection of Human PRMT6 on HeLa whole cell lysate using NB110-40713. PRMT6 was also immunoprecipitated by rabbit anti-PRMT6 antibody NB110-40712.
Immunohistochemistry-Paraffin: PRMT6 Antibody [NB110-40713]

Immunohistochemistry-Paraffin: PRMT6 Antibody [NB110-40713]

Immunohistochemistry-Paraffin: PRMT6 Antibody [NB110-40713] - Human breast carcinoma. Antibody: Affinity purified rabbit anti- PRMT6 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB

Applications for PRMT6 Antibody

Application
Recommended Usage

Immunohistochemistry

1:2000 - 1:10000

Immunohistochemistry-Paraffin

1:2000 - 1:10000

Western Blot

1:2000-1:10000
Application Notes
Immunoprecipitation is not recommended, use NB 110-40712. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PRMT6

PRMT6 severely impairs the function of HIV-1 Rev. PRMT6 is a protein arginine N-methyltransferase, and catalyzes the sequential transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation is a prevalent post-translational modification in eukaryotic cells that has been implicated in signal transduction, the metabolism of nascent pre-RNA, and the transcriptional activation processes. In addition, PRMT6 displays automethylation activity; it is the first PRMT to do so. PRMT6 has been shown to act as a restriction factor for HIV replication.

Long Name

Protein arginine N-methyltransferase 6

Alternate Names

HRMT1L6

Entrez Gene IDs

55170 (Human)

Gene Symbol

PRMT6

UniProt

AAK85733.1 (Human)

Additional PRMT6 Products

Product Documents for PRMT6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PRMT6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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