PRMT7 Antibody (JE64-78)

Western Blot: PRMT7 Antibody (JE64-78) [NBP3-32855]

Western Blot: PRMT7 Antibody (JE64-78) [NBP3-32855] - Western blot analysis of PRMT7 on different lysates with Rabbit anti-PRMT7 antibody (NBP3-32855) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (15 ug/Lane)
Lane 2: Jurkat cell lysate (15 ug/Lane)
Lane 3: HepG2 cell lysate (15 ug/Lane)
Lane 4: HEK-293 cell lysate (15 ug/Lane)
Lane 5: 293T cell lysate (15 ug/Lane)
Lane 6: MCF7 cell lysate (15 ug/Lane)
Lane 7: NIH/3T3 cell lysate (15 ug/Lane)
Lane 8: Mouse testis tissue lysate (30 ug/Lane)
Lane 9: Rat testis tissue lysate (30 ug/Lane)

Predicted band size: 78 kDa
Observed band size: 78 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32855) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: PRMT7 Antibody (JE64-78) [NBP3-32855]

Immunohistochemistry: PRMT7 Antibody (JE64-78) [NBP3-32855] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-PRMT7 antibody (NBP3-32855) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32855) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.