PRMT7 Antibody (PCRP-PRMT7-1A4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13741

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PCRP-PRMT7-1A4

Concentration

0.2 mg/ml

View all PRMT7 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human PRMT7 protein (Uniprot: Q9NVM4)

Reactivity Notes

Predicted to react with Rat.

Localization

Nucleus, Nucleoplasm, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

78.46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14144)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for PRMT7 Antibody (PCRP-PRMT7-1A4)

Western Blot: PRMT7 Antibody (PCRP-PRMT7-1A4) [NBP3-13741]

Western Blot: PRMT7 Antibody (PCRP-PRMT7-1A4) [NBP3-13741]

Western Blot: PRMT7 Antibody (PCRP-PRMT7-1A4) [NBP3-13741] - Western blot analysis of HeLa cell lysate using PRMT7 antibody (PCRP-PRMT7-1A4).
Immunocytochemistry/ Immunofluorescence: PRMT7 Antibody (PCRP-PRMT7-1A4) [NBP3-13741]

Immunocytochemistry/ Immunofluorescence: PRMT7 Antibody (PCRP-PRMT7-1A4) [NBP3-13741]

Immunocytochemistry/Immunofluorescence: PRMT7 Antibody (PCRP-PRMT7-1A4) [NBP3-13741] - Immunofluorescence Analysis of PFA-fixed U87 cells stained using PRMT7 Antibody (PCRP-PRMT7-1A4) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.
Flow Cytometry: PRMT7 Antibody (PCRP-PRMT7-1A4) [NBP3-13741]

Flow Cytometry: PRMT7 Antibody (PCRP-PRMT7-1A4) [NBP3-13741]

Flow Cytometry: PRMT7 Antibody (PCRP-PRMT7-1A4) [NBP3-13741] - Flow Cytometric Analysis of PFA-fixed HeLa cells. PRMT7 antibody (PCRP-PRMT7-1A4) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
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Applications for PRMT7 Antibody (PCRP-PRMT7-1A4)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PRMT7

Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing (Miranda et al., 2004 [PubMed 15044439]).[supplied by OMIM]

Long Name

Protein arginine N-methyltransferase 7

Alternate Names

EC 2.1.1.321, KIAA1933

Gene Symbol

PRMT7

UniProt

Q9NVM4 (Human)

Additional PRMT7 Products

Product Documents for PRMT7 Antibody (PCRP-PRMT7-1A4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PRMT7 Antibody (PCRP-PRMT7-1A4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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