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PRMT7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19939

Novus Biologicals, part of Bio-Techne
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NBP2-19939

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRMT7. The exact sequence is proprietary.

Reactivity Notes

Bovine (83%)

Localization

Cytoplasm; cytosol; Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PRMT7 Antibody

Western Blot: PRMT7 Antibody [NBP2-19939]

Western Blot: PRMT7 Antibody [NBP2-19939]

Western Blot: PRMT7 Antibody [NBP2-19939] - Sample (30 ug of whole cell lysate) A: PC-3 7. 5% SDS PAGE gel, diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: PRMT7 Antibody [NBP2-19939]

Immunocytochemistry/ Immunofluorescence: PRMT7 Antibody [NBP2-19939]

Immunocytochemistry/Immunofluorescence: PRMT7 Antibody [NBP2-19939] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRMT7 protein stained by PRMT7 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: PRMT7 Antibody [NBP2-19939]

Immunohistochemistry-Paraffin: PRMT7 Antibody [NBP2-19939]

Immunohistochemistry-Paraffin: PRMT7 Antibody [NBP2-19939] - Analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.

Applications for PRMT7 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Application Notes
ChIP- Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PRMT7

Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing (Miranda et al., 2004 [PubMed 15044439]).[supplied by OMIM]

Long Name

Protein arginine N-methyltransferase 7

Alternate Names

EC 2.1.1.321, KIAA1933

Gene Symbol

PRMT7

UniProt

Additional PRMT7 Products

Product Documents for PRMT7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PRMT7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.