proBDNF Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-98754

Novus Biologicals, part of Bio-Techne
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NB100-98754
NB100-98754-0.1mg
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all proBDNF Antibodies »

Product Specifications

Immunogen

A synthetic peptide from rat proBDNF conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and zebrafish and shares 96% identity with human sequence.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for proBDNF Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: proBDNF Antibody - Azide and BSA Free [NB100-98754]

Immunocytochemistry/ Immunofluorescence: proBDNF Antibody - Azide and BSA Free [NB100-98754]

Immunocytochemistry/Immunofluorescence: proBDNF Antibody [NB100-98754] - IF on rat hypophysis at 40 ug/ml concentration using Sheep antibody to rat, mouse, zebra fish proBDNF (pro BDNF, pro Brain-derived neurotrophic factor): IgG, DAPI counterstained.
Immunocytochemistry/ Immunofluorescence: proBDNF Antibody - Azide and BSA Free [NB100-98754]

Immunocytochemistry/ Immunofluorescence: proBDNF Antibody - Azide and BSA Free [NB100-98754]

Immunocytochemistry/Immunofluorescence: proBDNF Antibody [NB100-98754] - Rat hypophysis DAPI stained.

Applications for proBDNF Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

10-50 ug/ml

Immunohistochemistry-Paraffin

10-50 ug/ml

Western Blot

10-50 ug/ml

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Reconstitution

Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.

Formulation

Lyophilized from PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: proBDNF

FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted.

Alternate Names

Abrineurin, brain-derived neurotrophic factor, MGC34632, neurotrophin

Entrez Gene IDs

12064 (Mouse); 24225 (Rat)

Gene Symbol

BDNF

UniProt

P23560

Additional proBDNF Products

Product Documents for proBDNF Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for proBDNF Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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