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Prodynorphin/PDYN Antibody (2E12)

Novus Biologicals, part of Bio-Techne | Catalog # H00005173-M01

Novus Biologicals, part of Bio-Techne
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H00005173-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Prodynorphin/PDYN (NP_077722.1, 205 a.a. ~ 254 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KRYGGFLRRIRPKLKWDNQKRYGGFLRRQFKVVTRSQEDPNAYSGELFDA

Specificity

PDYN - prodynorphin (2E12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Prodynorphin/PDYN Antibody (2E12)

Western Blot: Prodynorphin/PDYN Antibody (2E12) [H00005173-M01]

Western Blot: Prodynorphin/PDYN Antibody (2E12) [H00005173-M01]

Western Blot: Prodynorphin/PDYN Antibody (2E12) [H00005173-M01] - Analysis of Prodynorphin/PDYN expression in transfected 293T cell line by Prodynorphin/PDYN monoclonal antibody (M01), clone 2E12.Lane 1: Prodynorphin/PDYN transfected lysate(28.4 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: Prodynorphin/PDYN Antibody (2E12) [H00005173-M01]

Immunoprecipitation: Prodynorphin/PDYN Antibody (2E12) [H00005173-M01]

Immunoprecipitation: Prodynorphin/PDYN Antibody (2E12) [H00005173-M01] - Analysis of Prodynorphin/PDYN transfected lysate using anti-Prodynorphin/PDYN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with Prodynorphin/PDYN MaxPab rabbit polyclonal antibody.
Sandwich ELISA: Prodynorphin/PDYN Antibody (2E12) [H00005173-M01] - Detection limit for recombinant GST tagged Prodynorphin/PDYN is 0.1 ng/ml as a capture antibody.

Applications for Prodynorphin/PDYN Antibody (2E12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using H00005173-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Prodynorphin/PDYN

The protein encoded by this gene is a preproprotein that is proteolytically processed to form the secreted opioid peptides beta-neoendorphin, dynorphin, leu-enkephalin, rimorphin, and leumorphin. These peptides are ligands for the kappa-type of opioid receptor. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]

Alternate Names

ADCA, Beta-neoendorphin-dynorphin, leu-enkephalin, leumorphin, neoendorphin-dynorphin-enkephalin prepropeptide, PENKB, preprodynorphin, preproenkephalin B, proenkephalin-B, rimorphin, SCA23

Entrez Gene IDs

5173 (Human)

Gene Symbol

PDYN

Additional Prodynorphin/PDYN Products

Product Documents for Prodynorphin/PDYN Antibody (2E12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Prodynorphin/PDYN Antibody (2E12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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