Profilin 1 Antibody (2Z1M4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16780

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16780-100ul
NBP3-16780-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2Z1M4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Profilin 1 Antibodies »

Product Summary for Profilin 1 Antibody (2Z1M4)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 61-140 of human PFN1 (P07737). VNGLTLGGQKCSVIRDSLLQDGEFSMDLRTKSTGGAPTFNVTVTKTDKTLVLLMGKEGVHGGLINKKCYEMASHLRRSQY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Profilin 1 Antibody (2Z1M4)

Western Blot: Profilin 1 Antibody (2Z1M4) [NBP3-16780]

Western Blot: Profilin 1 Antibody (2Z1M4) [NBP3-16780]

Western Blot: Profilin 1 Antibody (2Z1M4) [NBP3-16780] - Western blot analysis of extracts of various cell lines, using Profilin 1 Rabbit mAb (NBP3-16780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for Profilin 1 Antibody (2Z1M4)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Profilin 1

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role both in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels. It is also in expressed in skeletal muscle, the uterus and kidneys. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

Alternate Names

PFN1, PROF1, Profilin I

Gene Symbol

PFN1

Additional Profilin 1 Products

Product Documents for Profilin 1 Antibody (2Z1M4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Profilin 1 Antibody (2Z1M4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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