Profilin 1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23341
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Profilin 1 Antibody
Immunogen
Profilin synthetic peptide (coupled to carrier protein) that includes amino acids from the C-terminal region of human Profilin 1. This sequence is well conserved in rat and mouse Profilin 1, has some homology to Profilin-2a and Profilin-2b, and low homology to Profiin-3 and Profilin-4. [UniProt# P07737]
Specificity
The antibody detects a 15 kDa band corresponding to the apparent molecular mass of Profilin 1 in human Jurkat and A431 cells, as well as rat PC12 cells.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for Profilin 1 Antibody
Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody [NBP3-23341] -
Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody [NBP3-23341] - Immunocytochemical labeling of Profilin in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-Profilin-1 (NBP3-23341) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.
Western Blot: Profilin 1 Antibody [NBP3-23341] -
Western Blot: Profilin 1 Antibody [NBP3-23341] - Western blot of Jurkat stimulated with calyculin A (100 nM) for 30 min (lanes 1-6). The blots were probed with anti-Profilin (a.a. 132-140) (lanes 1-3) or anti-Profilin (Ser-138) phospho-specific (lanes 4-6). Both antibodies were used in the absence (lanes 1 & 4) or presence of unphosphorylated Profilin (Ser-138) (lanes 2 & 5) or phospho-Profilin (Ser-138) (lanes 3 & 6) blocking peptides.Applications for Profilin 1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Profilin 1
Alternate Names
PFN1, PROF1, Profilin I
Gene Symbol
PFN1
Additional Profilin 1 Products
Product Documents for Profilin 1 Antibody
Product Specific Notices for Profilin 1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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