Profilin 1 [p Tyr129] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23345
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Profilin 1 [p Tyr129] Antibody
Immunogen
Phospho-Profilin (Tyr-129) synthetic peptide (coupled to carrier protein) that includes amino acids surrounding Tyr-129 in human Profilin 1. This sequence is well conserved in rat and mouse Profilin 1. The tyrosine site has some homology to the conserved site (Tyr-129) in Profilin-2a and Profilin-2b, but the site is not conserved in Profiin-3 and Profilin-4. [UniProt# P07737]
Modification
p Tyr129
Specificity
This antibody was cross-adsorbed to unphosphorylated Profilin 1 (Tyr-129) then affinity purified using Profilin 1 (Tyr-129) peptide (without carrier). The antibody detects a 15 kDa band corresponding to the apparent molecular mass of Profilin 1 in human umbilical vein endothelial cells (HUVEC) and jurkat cells treated with pervanadate. This reactivity is removed by alkaline phosphatase treatment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for Profilin 1 [p Tyr129] Antibody
![Profilin 1 [p Tyr129] Antibody](https://resources.bio-techne.com/images/products/nbp3-23345_rabbit-profilin-1-p-tyr129-pab-19920231856966.jpg "Western Blot: Profilin 1 [p Tyr129] Antibody [NBP3-23345] -")
Western Blot: Profilin 1 [p Tyr129] Antibody [NBP3-23345] -
Western Blot: Profilin 1 [p Tyr129] Antibody [NBP3-23345] - Western blot of HUVEC stimulated with Pervanadate (1 mM) for 30 min (lanes 1-4). The blots were treated with alkaline phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Profilin (a.a. 126-137) (lanes 1 & 2) or anti-Profilin (Tyr-129) phospho-specific (lanes 3 & 4). The antibodies detect profilin at 15 kDa. In lanes 3 & 4, the antibody also detects an unknown 20 kDa protein.Applications for Profilin 1 [p Tyr129] Antibody
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Profilin 1
Alternate Names
PFN1, PROF1, Profilin I
Gene Symbol
PFN1
Additional Profilin 1 Products
Product Documents for Profilin 1 [p Tyr129] Antibody
Product Specific Notices for Profilin 1 [p Tyr129] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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