Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # PR484

Concentration

0.2 mg/ml

View all Progesterone R/NR3C3 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Progesterone R/NR3C3 protein (PGR) (Uniprot: P06401 )

Localization

Nucleus

Specificity

This monoclonal antibody is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa). Expression of PgR has been suggested to reflect a intact estrogen regulatory machinery and therefore, predict better clinical response to endocrine therapy than ER alone. It is excellent for immunohistochemical staining of formalin/paraffin tissues.

Marker

Marker of Progestin Dependence

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33325)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Progesterone R/NR3C3 Antibody (PR484)

Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (PR484) [NBP2-33324]

Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (PR484) [NBP2-33324]

Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (PR484) [NBP2-33324] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with Progesterone R/NR3C3 Mouse Monoclonal Antibody (PR484).
Simple Western: Progesterone R/NR3C3 Antibody (PR484) [NBP2-33324]

Simple Western: Progesterone R/NR3C3 Antibody (PR484) [NBP2-33324]

Simple Western: Progesterone R/NR3C3 Antibody (PR484) [NBP2-33324] - Simple Western lane view shows a specific band for Progesterone R/NR3C3 in 0.2 mg/ml of T47d (left) and MCF-7 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: Progesterone R/NR3C3 Antibody (PR484) [NBP2-33324]

Simple Western: Progesterone R/NR3C3 Antibody (PR484) [NBP2-33324]

Simple Western: Progesterone R/NR3C3 Antibody (PR484) [NBP2-33324] - Electropherogram image of the corresponding Simple Western lane. Progesterone R/NR3C3 antibody was used at 10 ug/ml dilution of T47d and MCF-7 lysates(s) respectively.

Applications for Progesterone R/NR3C3 Antibody (PR484)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Simple Western

10 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Progesterone R/NR3C3

The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription.

Long Name

Progesterone Receptor

Alternate Names

NR3C3, PGR, ProgesteroneR

Gene Symbol

PGR

UniProt

P06401

Additional Progesterone R/NR3C3 Products

Product Documents for Progesterone R/NR3C3 Antibody (PR484)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Progesterone R/NR3C3 Antibody (PR484)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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