Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Orangutan (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Prohibitin 2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Repressor of Estrogen Activity (Prohibitin 2) using the numbering given in entry NP_009204.1 (GeneID 11331).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Prohibitin 2 Antibody

Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NB100-1807]

Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NB100-1807]

Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NB100-1807] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- REA used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: Prohibitin 2 Antibody [NB100-1807]

Immunoprecipitation: Prohibitin 2 Antibody [NB100-1807]

Immunoprecipitation: Prohibitin 2 Antibody [NB100-1807] - Detection of Human REA by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg/IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-REA antibodies NB100-1807, another REA Ab, NB100-1808, and NB100-1809 each used for IP at 3 mcg/mg lysate. Immunoprecipitated REA was blotted using BL1706 (upper blot) or BL1707 (lower blot). Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower

Applications for Prohibitin 2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000- 1:5000

Immunohistochemistry-Paraffin

1:1000- 1:5000

Immunoprecipitation

1-4 ug/mg of lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Western Blot Not recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Prohibitin 2

REA (repressor of estrogen receptor activity), also known as prohibitin 2, has been shown to exhibit a variety of functions that includes mitochondrial chaperone activity, cell cycle regulation, and transcriptional regulation. REA localizes to the mitochondria and associates with mitochondrial complex I where it may play a role in mitochondrial metabolism. REA interacts with pRb to inhibit E2F-mediated transcription as well as the estrogen receptor to repress estrogen-dependent transcription via recruitment of histone deacetylases. Alternate names for REA include B-cell receptor-associated protein BAP37, D-prohibitin, PHB2, BAP, p22, BCAP37, PNAS-141, and MGC117268.

Alternate Names

BAP, Bap37, BCAP37, D-Prohibitin, p22, PHB2, PNAS-141, REA

Entrez Gene IDs

11331 (Human)

Gene Symbol

PHB2

UniProt

Q99623

Additional Prohibitin 2 Products

Product Documents for Prohibitin 2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Prohibitin 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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