Prohibitin 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-98312

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all Prohibitin 2 Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody is prohibitin 2 - middle region. Peptide sequence REYTAAVEAKQVAQQEAQRAQFLVEKAKQEQRQKIVQAEGEAEAAKMLGE. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Prohibitin 2 Antibody

Western Blot: Prohibitin 2 Antibody [NBP1-98312]

Western Blot: Prohibitin 2 Antibody [NBP1-98312]

Western Blot: Prohibitin 2 Antibody [NBP1-98312] - WB Suggested Anti-Phb2 Antibody. Positive Control: Lane 1: 80ug mouse brain extractLane 2: 80ug rat brain extract. Primary Antibody Dilution : 1:500. Secondary Antibody : IRDye 800 CW goat anti-rabbit from Li-COR Bioscience.
Western Blot: Prohibitin 2 Antibody [NBP1-98312]

Western Blot: Prohibitin 2 Antibody [NBP1-98312]

Western Blot: Prohibitin 2 Antibody [NBP1-98312] - Mouse Heart Lysate 1.0ug/ml, Gel Concentration: 12%
Immunoprecipitation: Prohibitin 2 Antibody [NBP1-98312]

Immunoprecipitation: Prohibitin 2 Antibody [NBP1-98312]

Immunoprecipitation: Prohibitin 2 Antibody [NBP1-98312] - NT2 CELL/BRAIN TISSUE

Applications for Prohibitin 2 Antibody

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Prohibitin 2

REA, or repressor of estrogen receptor activity, (also known as BAP37, or B-cell receptor-associated protein) is a 37 kD erythrocyte band 7 integral membrane protein that contains an SPFH domain. It is located in the inner mitochondrial membrane that selectively potentiates the inhibitory effectiveness of antiestrogen-occupied ER. REA plays a possible role in cell aging and acts as a mitochondrial protein chaperone. This protein has been shown to interact with the estrogen receptor, prohibitin, and IgM.

Alternate Names

BAP, Bap37, BCAP37, D-Prohibitin, p22, PHB2, PNAS-141, REA

Gene Symbol

PHB2

UniProt

NP_031557 (Mouse)

Additional Prohibitin 2 Products

Product Documents for Prohibitin 2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Prohibitin 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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