Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA Detection

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 204

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Prolactin R Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Prolactin R (Accession#: NP_000940.1; Met 1-Asp 234).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Prolactin R Antibody (204)

Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (204) [NBP2-89377]

Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (204) [NBP2-89377]

Immunocytochemistry/Immunofluorescence: Prolactin R Antibody (204) [NBP2-89377] - Staining of Human PRLR in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PRLR monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.f
Flow Cytometry: Prolactin R Antibody (204) [NBP2-89377]

Flow Cytometry: Prolactin R Antibody (204) [NBP2-89377]

Flow Cytometry: Prolactin R Antibody (204) [NBP2-89377] - Analysis of human PRLR expression on MCF-7 cells. Cells were stained with purified anti-Human PRLR, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.

Applications for Prolactin R Antibody (204)

Application
Recommended Usage

ELISA

1:5000-1:10000
Application Notes
This antibody will detect Human Prolactin R in ELISA pair set (Catalog: # NBP2-79554).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Prolactin R

Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

Long Name

Prolactin Receptor

Alternate Names

PRLR, ProlactinR

Gene Symbol

PRLR

Additional Prolactin R Products

Product Documents for Prolactin R Antibody (204)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Prolactin R Antibody (204)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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