Proliferation Marker Antibody (JC1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07762
![Immunohistochemistry-Paraffin: Proliferation Marker Antibody (JC1) [NBP3-07762]](https://resources.bio-techne.com/images/products/Proliferation-Marker-Antibody-JC1-Immunohistochemistry-Paraffin-NBP3-07762-img0002.jpg "Immunohistochemistry-Paraffin: Proliferation Marker Antibody (JC1) [NBP3-07762]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # JC1
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Pokeweed-stimulated lymphocytes
Localization
Nucleus
Specificity
JC1 detects a nuclear antigen, distinct from Ki67, present in proliferating cells. It is useful as a marker of proliferation in cases such as squamous cell carcinoma where the Ki67 index cannot be determined.
Marker
Proliferation Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08342)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Proliferation Marker Antibody (JC1)
Immunohistochemistry-Paraffin: Proliferation Marker Antibody (JC1) [NBP3-07762]
Immunohistochemistry-Paraffin: Proliferation Marker Antibody (JC1) [NBP3-07762] - Formalin-fixed, paraffin-embedded human Lymph Node stained with Proliferation Marker Mouse Monoclonal Antibody (JC1).![Immunohistochemistry-Paraffin: Proliferation Marker Antibody (JC1) [NBP3-07762]](https://resources.bio-techne.com/images/products/Proliferation-Marker-Antibody-JC1-Immunohistochemistry-Paraffin-NBP3-07762-img0001.jpg "Immunohistochemistry-Paraffin: Proliferation Marker Antibody (JC1) [NBP3-07762]")
Immunohistochemistry-Paraffin: Proliferation Marker Antibody (JC1) [NBP3-07762]
Immunohistochemistry-Paraffin: Proliferation Marker Antibody (JC1) [NBP3-07762] - Formalin-fixed, paraffin-embedded human Lymph Node stained with Proliferation Marker Mouse Monoclonal Antibody (JC1).Applications for Proliferation Marker Antibody (JC1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Proliferation Marker
Additional Proliferation Marker Products
Product Documents for Proliferation Marker Antibody (JC1)
Product Specific Notices for Proliferation Marker Antibody (JC1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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