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Prostaglandin I2 Synthase Antibody (3B11)

Novus Biologicals, part of Bio-Techne | Catalog # H00005740-M02

Novus Biologicals, part of Bio-Techne
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H00005740-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

PTGIS (NP_000952, 391 a.a. ~ 500 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PQRDPEIYTDPEVFKYNRFLNPDGSEKKDFYKDGKRLKNYNMPWGAGHNHCLGRSYAVNSIKQFVFLVLVHLDLELINADVEIPEFDLSRYGFGLMQPEHDVPVRYRIRP

Specificity

Reacts with prostaglandin I2 (prostacyclin) synthase.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Prostaglandin I2 Synthase Antibody (3B11)

Western Blot: Prostaglandin I2 Synthase Antibody (3B11) [H00005740-M02]

Western Blot: Prostaglandin I2 Synthase Antibody (3B11) [H00005740-M02]

Western Blot: Prostaglandin I2 Synthase Antibody (3B11) [H00005740-M02] - Analysis of PTGIS expression in transfected 293T cell line by PTGIS monoclonal antibody (M02), clone 3B11.Lane 1: PTGIS transfected lysate (Predicted MW: 57.1 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: Prostaglandin I2 Synthase Antibody (3B11) [H00005740-M02] - Detection limit for recombinant GST tagged PTGIS is 0.3 ng/ml as a capture antibody.

Applications for Prostaglandin I2 Synthase Antibody (3B11)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in WB and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Prostaglandin I2 Synthase

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to prostacyclin (prostaglandin I2), a potent vasodilator and inhibitor of platelet aggregation. An imbalance of prostacyclin and its physiological antagonist thromboxane A2 contribute to the development of myocardial infarction, stroke, and atherosclerosis. [provided by RefSeq]

Alternate Names

CYP8, CYP8A1, PGIS, Prostacyclin Synthase, PTGI

Entrez Gene IDs

5740 (Human)

Gene Symbol

PTGIS

Additional Prostaglandin I2 Synthase Products

Product Documents for Prostaglandin I2 Synthase Antibody (3B11)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Prostaglandin I2 Synthase Antibody (3B11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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