Protease Inhibitor 15 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82445
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (92%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Protease Inhibitor 15 Antibody
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LLFSLLCEASTVVLLNSTDSSPPTNNFTDIEAALKAQLDSADIPKARRKRYISQNDMIAILDYHNQVRGKVFPPAANMEYMVWDENLAKSAEAWAATCIWDHGPSYLLRFLGQNLSVRTGRYRSILQLVKPWYDEVKDY
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Protease Inhibitor 15 Antibody
![Western Blot: Protease Inhibitor 15 Antibody [NBP1-82445]](https://resources.bio-techne.com/images/products/Protease-Inhibitor-15-Antibody-Western-Blot-NBP1-82445-img0006.jpg "Western Blot: Protease Inhibitor 15 Antibody [NBP1-82445]")
Western Blot: Protease Inhibitor 15 Antibody [NBP1-82445]
Western Blot: Protease Inhibitor 15 Antibody [NBP1-82445] - Analysis in control (vector only transfected HEK293T lysate) and PI15 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunohistochemistry-Paraffin: Protease Inhibitor 15 Antibody [NBP1-82445]](https://resources.bio-techne.com/images/products/Protease-Inhibitor-15-Antibody-Immunohistochemistry-Paraffin-NBP1-82445-img0002.jpg "Immunohistochemistry-Paraffin: Protease Inhibitor 15 Antibody [NBP1-82445]")
Immunohistochemistry-Paraffin: Protease Inhibitor 15 Antibody [NBP1-82445]
Immunohistochemistry-Paraffin: Protease Inhibitor 15 Antibody [NBP1-82445] - Staining of human lung shows strong cytoplasmic positivity in alveolar macrophages.Applications for Protease Inhibitor 15 Antibody
Application
Recommended Usage
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Protease Inhibitor 15
Alternate Names
25 kDa trypsin inhibitor, CRISP8, CRISP-8, Cysteine-rich secretory protein 8, P24TI, p25TI, P25TIDKFZp686F0366, peptidase inhibitor 15, PI-15, protease inhibitor 15, sugarCrisp
Gene Symbol
PI15
Additional Protease Inhibitor 15 Products
Product Documents for Protease Inhibitor 15 Antibody
Product Specific Notices for Protease Inhibitor 15 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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