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Protease Inhibitor 15 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82445

Novus Biologicals, part of Bio-Techne
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NBP1-82445

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLFSLLCEASTVVLLNSTDSSPPTNNFTDIEAALKAQLDSADIPKARRKRYISQNDMIAILDYHNQVRGKVFPPAANMEYMVWDENLAKSAEAWAATCIWDHGPSYLLRFLGQNLSVRTGRYRSILQLVKPWYDEVKDY

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Protease Inhibitor 15 Antibody

Western Blot: Protease Inhibitor 15 Antibody [NBP1-82445]

Western Blot: Protease Inhibitor 15 Antibody [NBP1-82445]

Western Blot: Protease Inhibitor 15 Antibody [NBP1-82445] - Analysis in control (vector only transfected HEK293T lysate) and PI15 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Protease Inhibitor 15 Antibody [NBP1-82445]

Immunohistochemistry-Paraffin: Protease Inhibitor 15 Antibody [NBP1-82445]

Immunohistochemistry-Paraffin: Protease Inhibitor 15 Antibody [NBP1-82445] - Staining of human lung shows strong cytoplasmic positivity in alveolar macrophages.

Applications for Protease Inhibitor 15 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Protease Inhibitor 15

Protease Inhibitor 15 encodes a trypsin inhibitor. The protein shares similarity to insect venom allergens, mammalian testis-specific proteins and plant pathogenesis-related proteins. It is frequently expressed in human neuroblastoma and glioblastoma cell lines, and thus may play a role in the central nervous system.

Alternate Names

25 kDa trypsin inhibitor, CRISP8, CRISP-8, Cysteine-rich secretory protein 8, P24TI, p25TI, P25TIDKFZp686F0366, peptidase inhibitor 15, PI-15, protease inhibitor 15, sugarCrisp

Gene Symbol

PI15

Additional Protease Inhibitor 15 Products

Product Documents for Protease Inhibitor 15 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Protease Inhibitor 15 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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