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Protease Inhibitor 15 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-11581

Novus Biologicals, part of Bio-Techne
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NBP2-11581

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-RTGRYRSILQLVKP corresponding to internal region according to NP_056970.1.

Epitope

CRTGRYRSILQLVKP

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Protease Inhibitor 15 Antibody

Western Blot: Protease Inhibitor 15 Antibody [NBP2-11581]

Western Blot: Protease Inhibitor 15 Antibody [NBP2-11581]

Western Blot: Protease Inhibitor 15 Antibody [NBP2-11581] - HeLa lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Protease Inhibitor 15 Antibody [NBP2-11581]

Immunohistochemistry-Paraffin: Protease Inhibitor 15 Antibody [NBP2-11581]

Immunohistochemistry-Paraffin: Protease Inhibitor 15 Antibody [NBP2-11581] - Human Skeletal Muscle. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Protease Inhibitor 15 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:1000

Western Blot

0.3 - 1 ug/mL
Application Notes
WB: Approx. 33 kDa band observed in HeLa lysates (calculated MW of 29.1 kDa band according to NP_056970.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Protease Inhibitor 15

Protease Inhibitor 15 encodes a trypsin inhibitor. The protein shares similarity to insect venom allergens, mammalian testis-specific proteins and plant pathogenesis-related proteins. It is frequently expressed in human neuroblastoma and glioblastoma cell lines, and thus may play a role in the central nervous system.

Alternate Names

25 kDa trypsin inhibitor, CRISP8, CRISP-8, Cysteine-rich secretory protein 8, P24TI, p25TI, P25TIDKFZp686F0366, peptidase inhibitor 15, PI-15, protease inhibitor 15, sugarCrisp

Entrez Gene IDs

51050 (Human); 94227 (Mouse); 301489 (Rat)

Gene Symbol

PI15

Additional Protease Inhibitor 15 Products

Product Documents for Protease Inhibitor 15 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Protease Inhibitor 15 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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