Proteasome 19S 10B Antibody (OTI1G6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00990

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1G6

Concentration

0.7 mg/ml

View all Proteasome 19S 10B Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PSMD2(NP_002799) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Proteasome 19S 10B Antibody (OTI1G6)

Western Blot: Proteasome 19S 10B Antibody (OTI1G6) [NBP2-00990]

Western Blot: Proteasome 19S 10B Antibody (OTI1G6) [NBP2-00990]

Western Blot: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 19S 10B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 19S 10B.
Immunocytochemistry/ Immunofluorescence: Proteasome 19S 10B Antibody (OTI1G6) [NBP2-00990]

Immunocytochemistry/ Immunofluorescence: Proteasome 19S 10B Antibody (OTI1G6) [NBP2-00990]

Immunocytochemistry/Immunofluorescence: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Proteasome 19S 10B.
Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (OTI1G6) [NBP2-00990]

Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (OTI1G6) [NBP2-00990]

Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Human pancreas tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.
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Applications for Proteasome 19S 10B Antibody (OTI1G6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.7 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Proteasome 19S 10B

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. [provided by RefSeq].

Alternate Names

26S proteasome regulatory subunit RPN1, 26S proteasome regulatory subunit S2, MGC14274,55.11 protein, P97, proteasome (prosome, macropain) 26S subunit, non-ATPase, 2, Protein 55.11, Rpn1, S226S proteasome non-ATPase regulatory subunit 2, TNFR-associated protein 2, TRAP226S proteasome subunit p97, tumor necrosis factor receptor-associated protein 2, Tumor necrosis factor type 1 receptor-associated protein 2

Entrez Gene IDs

5708 (Human)

Gene Symbol

PSMD2

Additional Proteasome 19S 10B Products

Product Documents for Proteasome 19S 10B Antibody (OTI1G6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Proteasome 19S 10B Antibody (OTI1G6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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