Proteasome 19S S7 Antibody (4C10-2C8)

Novus Biologicals, part of Bio-Techne | Catalog # H00005701-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4C10-2C8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Proteasome 19S S7 Antibodies »

Product Specifications

Immunogen

PSMC2 (AAH02589.1, 1 a.a. ~ 433 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPDYLGADQRKTKEDEKDDKPIRALDEGDIALLKTYGQSTYSRQIKQVEDDIQQLLKKINELTGIKESDTGLAPPALWDLAADKQTLQSEQPLQVARCTKIINADSEDPKYIINVKQFAKFVVDLSDQVAPTDIEEGMRVGVDRNKYQIHIPLPPKIDPTVTMMQVEEKPDVTYSDVGGCKEQIEKLREVVETPLLHPERFVNLGIEPPKGVLLFGPPGTGKTLCARAVANRTDACFIRVIGSELVQKYVGEGARMVRELFEMARTKKACLIFFDEIDAIGGARFDDGAGGDNEVQRTMLELINQLDGFDPRGNIKVLMATNRPDTLDPALMRPGRLDRKIEFSLPDLEGRTHIFKIHARSMSVERDIRFELLARLCPNSTGAEIRSVCTEAGMFAIRARRKIATEKDFLEAVNKVIKSYAKFSATPRYMTYN

Localization

Cytoplasmic and Nuclear

Specificity

PSMC2 - proteasome (prosome, macropain) 26S subunit, ATPase, 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Proteasome 19S S7 Antibody (4C10-2C8)

Western Blot: Proteasome 19S S7 Antibody (4C10-2C8) [H00005701-M01]

Western Blot: Proteasome 19S S7 Antibody (4C10-2C8) [H00005701-M01]

Western Blot: Proteasome 19S S7 Antibody (4C10-2C8) [H00005701-M01] - PSMC2 monoclonal antibody (M01), clone 4C10-2C8 Analysis of PSMC2 expression in SW-13.
Immunocytochemistry/ Immunofluorescence: Proteasome 19S S7 Antibody (4C10-2C8) [H00005701-M01]

Immunocytochemistry/ Immunofluorescence: Proteasome 19S S7 Antibody (4C10-2C8) [H00005701-M01]

Immunocytochemistry/Immunofluorescence: Proteasome 19S S7 Antibody (4C10-2C8) [H00005701-M01] - Analysis of monoclonal antibody to PSMC2 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: Proteasome 19S S7 Antibody (4C10-2C8) [H00005701-M01] - Detection limit for recombinant GST tagged PSMC2 is 0.3 ng/ml as a capture antibody.

Applications for Proteasome 19S S7 Antibody (4C10-2C8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Proteasome 19S S7

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit has been shown to interact with several of the basal transcription factors so, in addition to participation in proteasome functions, this subunit may participate in the regulation of transcription. This subunit may also compete with PSMC3 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex.

Alternate Names

26S protease regulatory subunit 7, 26S proteasome AAA-ATPase subunit RPT1, MGC3004, MSS1ATPase, 2, proteasome (prosome, macropain) 26S subunit, ATPase, 2, Protein MSS1, putative protein product of Nbla10058

Entrez Gene IDs

5701 (Human)

Gene Symbol

PSMC2

OMIM

154365 (Human)

UniProt

AAH02589 (Human)

Additional Proteasome 19S S7 Products

Product Documents for Proteasome 19S S7 Antibody (4C10-2C8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Proteasome 19S S7 Antibody (4C10-2C8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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