Proteasome 20S alpha 5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB120-11437

Novus Biologicals, part of Bio-Techne
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NB120-11437
NB120-11437-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all Proteasome 20S alpha 5 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues C A(120) L Q F G E E D A D P G A M(133) of human proteasome 20S subunit alpha 5.

Localization

Cytoplasmic and Nuclear

Specificity

This detects proteasome 20S subunit alpha 5 from human, rat and mouse cell lysates.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Proteasome 20S alpha 5 Antibody

Western Blot: Proteasome 20S alpha 5 Antibody [NB120-11437]

Western Blot: Proteasome 20S alpha 5 Antibody [NB120-11437]

Western Blot: Proteasome 20S alpha 5 Antibody [NB120-11437] - Analysis of HeLa cell extract. Lane 1 is NB120-11437. Lane 2 is NB120-11437 pre-incubated with peptide.
Immunocytochemistry/ Immunofluorescence: Proteasome 20S alpha 5 Antibody [NB120-11437]

Immunocytochemistry/ Immunofluorescence: Proteasome 20S alpha 5 Antibody [NB120-11437]

Immunocytochemistry/Immunofluorescence: Proteasome 20S alpha 5 Antibody [NB120-11437] - HeLa cells

Applications for Proteasome 20S alpha 5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1 ug/ml

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Western Blot

1 ug/ml
Application Notes
WB: Detects an approx. 26.5 kDa protein.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Proteasome 20S alpha 5

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signaling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.

Long Name

Proteasome subunit alpha type-5

Alternate Names

PSMA5

Entrez Gene IDs

5686 (Human); 26442 (Mouse); 29672 (Rat)

Gene Symbol

PSMA5

Additional Proteasome 20S alpha 5 Products

Product Documents for Proteasome 20S alpha 5 Antibody

Product Datasheets

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Product Specific Notices for Proteasome 20S alpha 5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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