Proteasome 20S alpha 5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32387

Novus Biologicals, part of Bio-Techne
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NBP1-32387
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Proteasome 20S alpha 5 Antibodies »

Product Summary for Proteasome 20S alpha 5 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Proteasome 20S alpha 5. The exact sequence is proprietary.

Localization

Cytoplasm, Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Proteasome 20S alpha 5 Antibody

Western Blot: Proteasome 20S alpha 5 Antibody [NBP1-32387]

Western Blot: Proteasome 20S alpha 5 Antibody [NBP1-32387]

Western Blot: Proteasome 20S alpha 5 Antibody [NBP1-32387] - Proteasome 20S alpha 5 antibody detects Proteasome 20S alpha 5 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Proteasome 20S alpha 5 antibody diluted by 1:1000.
Immunocytochemistry/ Immunofluorescence: Proteasome 20S alpha 5 Antibody [NBP1-32387]

Immunocytochemistry/ Immunofluorescence: Proteasome 20S alpha 5 Antibody [NBP1-32387]

Immunocytochemistry/Immunofluorescence: Proteasome 20S alpha 5 Antibody [NBP1-32387] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Proteasome 20S alpha 5 Antibody [NBP1-32387]

Immunohistochemistry-Paraffin: Proteasome 20S alpha 5 Antibody [NBP1-32387]

Immunohistochemistry-Paraffin: Proteasome 20S alpha 5 Antibody [NBP1-32387] - Proteasome 20S alpha 5 antibody detects Proteasome 20S alpha 5 protein at cytoplasm and nucleus in human hepatocellular carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. Proteasome 20S alpha 5 antibody diluted at 1:500.
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Applications for Proteasome 20S alpha 5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Proteasome 20S alpha 5

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.

Long Name

Proteasome subunit alpha type-5

Alternate Names

PSMA5

Gene Symbol

PSMA5

Additional Proteasome 20S alpha 5 Products

Product Documents for Proteasome 20S alpha 5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Proteasome 20S alpha 5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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