Proteasome 20S beta 3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52079

Novus Biologicals, part of Bio-Techne
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NBP1-52079
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Proteasome 20S beta 3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-NLYELKEGRQ corresponding to internal region according to NP_002786.2.

Epitope

C-NLYELKEGRQ

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Proteasome 20S beta 3 Antibody

Western Blot: Proteasome 20S beta 3 Antibody [NBP1-52079]

Western Blot: Proteasome 20S beta 3 Antibody [NBP1-52079]

Western Blot: Proteasome 20S beta 3 Antibody [NBP1-52079] - (1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Proteasome 20S beta 3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 24 kDa band observed in K562 lysates (calculated MW of 22.9 kDa band according to NP_002786.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Proteasome 20S beta 3

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Pseudogenes have been identified on chromosomes 2 and 12.

Long Name

Proteasome subunit beta type-3

Alternate Names

Proteasome chain 13, PSMB3

Entrez Gene IDs

5691 (Human); 26446 (Mouse); 29676 (Rat)

Gene Symbol

PSMB3

UniProt

NP_002786.2 (Human)

Additional Proteasome 20S beta 3 Products

Product Documents for Proteasome 20S beta 3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Proteasome 20S beta 3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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