Protein A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53424
Key Product Details
Species Reactivity
Bacteria
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Protein A [Staphylococcus aureus]
Reactivity Notes
Cross reactivity against Protein A from other sources is unknown
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A [Staphylococcus aureus] and anti-Goat Serum
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Protein A Antibody
![Western Blot: Protein A Antibody [NBP2-53424]](https://resources.bio-techne.com/images/products/Protein-A-Antibody-Western-Blot-NBP2-53424-img0001.jpg "Western Blot: Protein A Antibody [NBP2-53424]")
Western Blot: Protein A Antibody [NBP2-53424]
Western Blot: Protein A Antibody [NBP2-53424] - Lane 1: Protein A Reduced. Lane 2: Protein A Reduced. Lane 3: Protein A Non-Reduced. Lane 4: Protein A Non-Reduced. Load: Lane 1 and 3 - 1.0 ug per lane, Lane 2 and 4 - 0.25 ug per lane. Primary antibody: Protein A antibody at 1:5,000 for overnight at 4C. Secondary antibody: DyLight 649 goat secondary antibody at 1:10,000 for 90 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: approx. 50 kDa for Protein A. Other band(s): Protein A splice variants and isoforms.
Protein A Antibody
Western Blot of Goat anti-Protein A Antibody. Lane 1: Protein A. Lane 2: None. Load: 50 ng per lane. Primary antibody: Protein A antibody at 1:1,000 overnight at 4C. Secondary antibody: IRDye800(R) goat secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 43 kDa, 53 kDa for Protein A. Other band(s): Protein A splice variants and isoforms.Applications for Protein A Antibody
Application
Recommended Usage
ELISA
1:20000-1:100000
Immunoprecipitation
1:1000-1:5000
Western Blot
1:2000-1:10000
Application Notes
This product has been tested by western blot and is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity. Optimal concentration in immunoassays should be determined by the researcher.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: Protein A
Alternate Names
PROA, Protein-A
Gene Symbol
SAOUHSC_00069
Additional Protein A Products
Product Documents for Protein A Antibody
Product Specific Notices for Protein A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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