Protein Disulfide Isomerase/P4HB Antibody (16)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89676

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 16

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Protein Disulfide Isomerase/P4HB Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Protein Disulfide Isomerase/P4HB (Accession#: NP_000909.2; Met 1-Lys 505).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Protein Disulfide Isomerase/P4HB Antibody (16)

Western Blot: Protein Disulfide Isomerase/P4HB Antibody (16) [NBP2-89676]

Western Blot: Protein Disulfide Isomerase/P4HB Antibody (16) [NBP2-89676]

Western Blot: Protein Disulfide Isomerase/P4HB Antibody (16) [NBP2-89676] - Lane A: MCF7 Whole Cell Lysate Lane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique.Performed under reducing conditions. Predicted band size:57 kDaObserved band size:57 kDa (We are unsure as to the identity of these extra bands.)
Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (16) [NBP2-89676]

Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (16) [NBP2-89676]

Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (16) [NBP2-89676] - Staining of Human P4HB in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human P4HB monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to endoplasmic reticulum.
Immunoprecipitation: Protein Disulfide Isomerase/P4HB Antibody (16) [NBP2-89676]

Immunoprecipitation: Protein Disulfide Isomerase/P4HB Antibody (16) [NBP2-89676]

Immunoprecipitation: Protein Disulfide Isomerase/P4HB Antibody (16) [NBP2-89676] - Lane A:0.5 mg MCF-7 Whole Cell Lysate 2 uL anti-P4HB rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-P4HB rabbit monoclonal antibody,at 1:200 dilution Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 57 kDa Observed band size: 57 kDa

Applications for Protein Disulfide Isomerase/P4HB Antibody (16)

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunoprecipitation

1-4 ul/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Protein Disulfide Isomerase/P4HB

P4HB, the beta subunit of prolyl 4-hydroxylase, a highly abundant member of the PDI family. P4HB is a multifunctional enzyme, involved in hydroxylation of prolyl residues in preprocollagen, as well as continual rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Long Name

Proline 4-Hydroxylase, Beta Polypeptide

Alternate Names

DSI, ERBA2L, GIT, PDI, PDIA1, PO4DB, PO4HB, PROHB, Thbp

Gene Symbol

P4HB

Additional Protein Disulfide Isomerase/P4HB Products

Product Documents for Protein Disulfide Isomerase/P4HB Antibody (16)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Protein Disulfide Isomerase/P4HB Antibody (16)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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