Protein Disulfide Isomerase/P4HB Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1921
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Primate
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Purified full length P4hb isolated from rat liver.
Localization
Endoplasmic reticulum
Marker
Endoplasmic Reticulum Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Protein Disulfide Isomerase/P4HB Antibody
Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NB100-1921] -
Normal human brain.
Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [NB100-1921] -
ICC/IF Confocal analysis of 3T3 cells using P4HB antibody (NB100-1921, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NB100-1921] -
IHC analysis of a formalin fixed paraffin-embedded (FFPE) human brain using 1:250 conc. of P4HB antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of P4HB in neurons was observed. Staining was performed by Histowiz.Applications for Protein Disulfide Isomerase/P4HB Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:5000-1:20000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Frozen
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
1-3 ug
Western Blot
1:10000-1:20000
Application Notes
This P4hb antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry frozen and paraffin embedded sections, Immunoprecipitation and Western Blot.
Reviewed Applications
Read 2 reviews rated 5 using NB100-1921 in the following applications:
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Whole antisera
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Protein Disulfide Isomerase/P4HB
Long Name
Proline 4-Hydroxylase, Beta Polypeptide
Alternate Names
DSI, ERBA2L, GIT, PDI, PDIA1, PO4DB, PO4HB, PROHB, Thbp
Gene Symbol
P4HB
Additional Protein Disulfide Isomerase/P4HB Products
Product Documents for Protein Disulfide Isomerase/P4HB Antibody
Product Specific Notices for Protein Disulfide Isomerase/P4HB Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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