Protein Kinase A regulatory subunit I alpha Antibody (S06-8A5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15086

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15086-100ul
NBP3-15086-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S06-8A5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Protein Kinase A regulatory subunit I alpha Antibodies »

Product Summary for Protein Kinase A regulatory subunit I alpha Antibody (S06-8A5)

Immunogen

A synthetic peptide of human Protein Kinase A regulatory subunit I alpha (Uniprot#: P10644)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Protein Kinase A regulatory subunit I alpha Antibody (S06-8A5)

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (S06-8A5) [NBP3-15086]

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (S06-8A5) [NBP3-15086]

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (S06-8A5) [NBP3-15086] - Western blot detection of Protein Kinase A regulatory subunit I alpha/PRKAR1A in C6, Rat Brain, Jurkat cell lysates using Protein Kinase A regulatory subunit I alpha/PRKAR1A Rabbit mAb (1:500 diluted). Predicted band size: 43kDa. Observed band size: 43kDa

Applications for Protein Kinase A regulatory subunit I alpha Antibody (S06-8A5)

Application
Recommended Usage

Western Blot

1/1000-1/5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Protein Kinase A regulatory subunit I alpha

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Three alternatively spliced transcript variants encoding the same protein have been observed.

Long Name

cAMP-dependent protein kinase type I-alpha regulatory subunit

Alternate Names

PRKAR1, PRKAR1A, TSE1

Gene Symbol

PRKAR1A

Additional Protein Kinase A regulatory subunit I alpha Products

Product Documents for Protein Kinase A regulatory subunit I alpha Antibody (S06-8A5)

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Certificate of Analysis

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Product Specific Notices for Protein Kinase A regulatory subunit I alpha Antibody (S06-8A5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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